Imaging Plate FC

Black multiwell plates with fluorcarbon film bottom (25µm)

Order information

         
Article number Wells / Plate  Plates/ bag Plates/ Sales Unit Lid Sterile
3221-20 6 1 / bag 20 /box + +
3231-20 24 1 / bag 20 / box + +
3241-20 96 1 / bag 20 / box + +


Features:

  • Gas permeable bottom
  • UV light transmission
  • Low skirt design, complete area access with immersion objectives
  • Tissue culture surface
  • Excellent planicity

Applications:

Life cell imaging, immunohistochemistry, super high resolution microscopy, laser scanning microscopy, high content analysis, hypoxia stidies, photoxicity studies

Detailed Description:

Imaging Plates FC are black multiwell plates compliant to the SBS (Society for Biomolecular Screening) standard-format with bottoms made from a 25 µm fluorcarbon film (FC). During the manufacturing the thin film is stretched arround each individual well bottom to provide tension and planicity. Because of the thin bottom and material properties the plates show excellent light transmission rates even for UV-A and UV-B light. The intrinsic fluorescence of the material is much lower if compared to conventional polystyrene bottoms with significant reduction of background signals. Furthermore the film bottom enables a high gas transfer through the film. Oxygen supply and equilibration with the atmosphere are achieved directly through the plate bottom.The plate bodies are made from polystyrene and tightly bonded to the FC film. Imaging Plates FC can be used for high resolution Live Cell Imaging. In addition direct fixation and staining within the plates is possible (formaldehyda, glutaraldehyde, alcohols and acetone can be used). Acetone should not be applied more that 10 minutes because the plate body is made from polystyrene and subsequent to the material reaction the film bottom may detach. Imaging Plates FC can be used between -20°C and 50°C. Due to the thin film  bottom the application of high resolution immersion objectives with high numerical aperture is possible. For optimal focus control when objectives with 40x magnification and higher the use of objectives with correction rings or collars is recommended. Water, oil and glcyerine can be used as immersion media.

 

Specification

Properties of fluorcarbon film

 
Thickness 25 µm +/- 10%
Film type High performance fluorcarbon film
Refractive index 1,34
Abbe's number 70
Light transmission @ 240 nm > 85%
Light transmission @ 300 nm > 90%
Oxygen transfer capacity >6300 cm3/(m2*d* bar)
Carbon dioxide transfer capacity >7000 cm3/(m2*d* bar)
Thermal transfer capacity 0.01 mW/K
Surface quality TC (Tissue Culture Quality)

 

TC= Tissue Culture, aminanted surface, suitable for most adherend cell lines (ze.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (z.B. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings

 

Technical data

     
Article number 3221 3231 3241
Format 6 well 24 well 96 well
Plate length 127,76 mm 127,76 mm 127,76 mm
Plate width 85,48 mm 85,48 mm 85,48 mm
Plate height 15 mm 15 mm 14,35 mm
Depth per well 14,6 mm 14,6 mm 13,95 mm
Well diameter (focal plane) 32 mm 13,2 mm 6 mm
Distance well center to well center 36 mm 18 mm 9 mm
Distance center of well A1 to longitudinal plate border 24,74 mm 15.74 mm 11.24 mm
Distance center of well A1 to short plate border 27,88 mm 18,88 mm 14,38 mm
Distance lower plate level (skirt rim) to focal plane 400 µm 400 µm 400 µm
Planicity per well < 10 µm < 10 µm < 10 µm
Planicity per plate < 50 µm < 50 µm < 50 µm
Total volume per well 12112 µl 1880 μl 428 µl
Suggested working volume peer well 2000-4000 µl 500-1000 μl

100-200 µl

 

All polymer plate bodies react to temperature shifts with dimensional changes. Please take care that the plate and it's environment have equal temperature during microscopy to minimze focal shifts and reduce refocussing..

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