Imaging Plate CG

Black multiwell plates with coverglass bottom (145 or 170µm thickness)

Order information

           
Article number Wells / Plate Coverglass thickness  Plates/ bag Plates/ Sales Unit Lid Sterile
5221-20 6 145 µm (1.0) 1 / bag 20 /box + +
5231-20 24 145 µm (1.0) 1 / bag 20 / box + +
5241-20 96 145 µm (1.0) 1 / bag 20 / box + +
5222-20 6 170 µm (1.5) 1 / bag 20 / box + +
5231-20 24 170 µm (1.5) 1 / bag 20 / box + +
5242-20 96 170 µm (1.5) 1 / bag 20 / box + +


Features:

  • Low skirt design, complete area access with immersion objectives
  • Tissue culture surface borosilicate cover glass
  • Excellent planicity

Applications:

Life cell imaging, immunohistochemistry, super high resolution microscopy, fluorecence correlation spectroscopy, laser scanning microscopy, high content analysis, 

Detailed Description:

Imaging Plates CG are black multiwell plates compliant to the SBS (Society for Biomolecular Screening) standard-format with cover glass bottoms made from borosilicate glass. The plate bodies are made from polystyrene and tightly bonded to the cover glass. Imaging Plates CG can be used for high resolution Live Cell Imaging. In addition direct fixation and staining within the plates is possible (formaldehyda, glutaraldehyde, alcohols and acetone can be used). Acetone should not be applied more that 10 minutes because the plate body is made from polystyrene and subsequent to the material reaction the cover glass may detach. Imaging Plates CG can be used between -20°C and 50°C. Due to the cover glass bottom the application of high resolution immersion objectives with high numerical aperture is possible. Water, oil and glcyerine can be used as immersion media.

Cell adhesion, spreading and distribution are supported by our approved plasma surface modification.  

Cover glass is still the best performing object carrier for high resolution light microscopy. Imaging Plate CG products are equipped with a bottom made from 145µm thick borosilicate glass. To support cell adhesion, spreading and equal distribution the glass is plasma modified to increase the amount of amino groups

All Imaging Plates have a black plate body to supress cross signals and to prevent excitation of regions out of interest in epifluorescence applications.

Alle Imaging Plates are single packed and shipped with a lid.
All lids contain condensation rings and spacers for optimal ventilation.

 

Specification

Cover glass properties

 
Thickness 1.0, 145 µm +/- 10%
Glass type borosilicate, hydrolytic class I
Refractive Index 1,52
Abbe's number 50
Light transmission @ 310 nm > 85%
Light transmission @ 360 nm > 90%
Surface quality TC (Tissue Culture Quality)

 

TC= Tissue Culture, aminanted surface, suitable for most adherend cell lines (ze.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (z.B. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.

 

Technical data

     
Article number 5221 / 5222 5231 / 5232 5241 / 5242
Format 6 well 24 well 96 well
Plate length 127,76 mm 127,76 mm 127,76 mm
Plate width 85,48 mm 85,48 mm 85,48 mm
Plate height 15 mm 15 mm 14,35 mm
Depth per well 14,6 mm 14,6 mm 13,95 mm
Well diameter (focal plane) 32 mm 13,2 mm 6 mm
Distance well center to well center 36 mm 18 mm 9 mm
Distance center of well A1 to longitudinal plate border 24,74 mm 15.74 mm 11.24 mm
Distance center of well A1 to short plate border 27,88 mm 18,88 mm 14,38 mm
Distance lower plate level (skirt rim) to focal plane 400 µm 400 µm 400 µm
Planicity per well < 10 µm < 10 µm < 10 µm
Planicity per plate < 50 µm < 50 µm < 50 µm
Total volume per well 12112 µl 1880 μl 428 µl
Suggested working volume peer well 2000-4000 µl 500-1000 μl

100-200 µl

 

All polymer plate bodies react to temperature shifts with dimensional changes. Please take care that the plate and it's environment have equal temperature during microscopy to minimze focal shifts and reduce refocussing..

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