Imaging Dish

  • Imaging Dish

    Imaging Dish

35 mm petri dish with central 18 mm diameter cover glass bottom

Order information

         
Article no. Cover glass thickness   Dishes/ Sales Unit Lid Sterile
5160-30 1.0, 145 µm 2 / Bag 30 / Box + +
5160-168 1.0, 145 µm 2 / Bag 168 / Box + +
6160-30 1.5, 170 µm 2 / Bag 30 / Box + +
6160-168 1.5, 170 µm 2 / Bag 168 / Box + +

 

Features:

  • Low skirt format: ideal for immersion microscopy
  • Robust format: stability against focal shift during temperature changes and time lapse studies
  • 12 notch grip zone: easy grip for dish and lid
  • Orientation marks: align dish on microscope stage
  • Imaging Reservoir: reduced volume for staining and labelling
  • Tissue Culture Surface treatment: improved cell spreading, adhesion and growth

 

Applications:

Life Cell Imaging, Fluorescence-Correlation spektroscopy (FCS), Confocal Laser Scanning Microscopy (LSM), Total Internal Reflection Fluorescence (TIRF), Differential Interference Contrast (DIC/Nomarski), Fluorescence Resonance Energy Transfer (FRET), Fluorecence Recovery after Photobleaching (FRAP), Weakly fluorescent stains / proteins, Fluorescence In-Situ Hybridisation (FISH), Immune histology

Detailed description: 

The Imaging Dish products are our entry solution for high resolution microscopy of living and fixed cells. The bottom of the 35mm Petridishes is made from cover glass. The central cover glass area has a diameter of 18mm. The level of the cover glass is 2mm lower than the basement level of the surrounding polystyrene dish. Due to this feature it is possible to concentrate the cells during the seeding on the glass surface.

Several features support a user friendly manipulation of the dish. The grip zone of the dish body is increased and allows a safe picking of the dish. The lid contains spacers for a good ventilation. For cell manipulations teh lid can easily be removed. The dishes are delivered steril in bags of six dishes. The dishes are placed on a tray of polystyrene which can be used for better transport of the dishes in the lab.

 

The focus level of the glass area is placed only 400µm above the basement line of the dish body. High quality cover glasses made from borosilicate glass are used. Variants with cover glass thickness 1.0 and 1.5 (145µm and 175µm ± 15µm repectively) are offered. These glass bottoms provide an excellent planicity and focus control and superior imaging qualities.

 

The macroscopic orientation of the dishes is supported by four marks (N-O-S-W). Variants exist (Imaging Dish µGridm 7160-30, 7160-168) with engraved microgrids to support microscopic orientation to retrieve the region of interest (ROI). The twelve corner grip zone of the dishes enables an easy orientation and positioning of the dies if combined with our Imaging Dish Stage Frame. Therfore the same orientation of the dishes can be established even if the dish has to be removed from the stage in between two observation sesions.

Specification

Cover glass properties

 
Thickness

1.0, 145 µm +/- 10%

1.5, 170 µm +/- 10%

Glass type borosilicate, hydrolytic class I
Refractive Index 1,52
Abbe's number 50
Light transmission @ 310 nm > 85%
Light transmission @ 360 nm > 90%
Surface quality TC (Tissue Culture Quality)

 

TC= Tissue Culture, aminanted surface, suitable for most adherend cell lines (ze.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (z.B. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.

 

Technical data

 
Article number 5160/6160
Outer dish diameter 35 mm
Diameter cover glass area 18 mm
Dish height 9,4 mm
Distance dish bottom rim to focus plane 400 µm
Planicity < 5 µm
Total dish volume 7 ml
Suggested working volume 2 ml
Suggested cell seeding volume 750 μl
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