Imaging Dish µGrid

  • Imaging Dish µGrid

    Imaging Dish µGrid

35 mm petri dish with central 18 mm diameter cover glass bottom with engraved µGrid

Order information

         
Article no. Cover glass thickness   Dishes/ Sales unit Lid Sterile
7160-30 1.5, 170 µm 2 / bag 30 / box + +
7160-168 1.5, 170 µm 2 / bag 168 / box + +

 

Features:

  • Laser engraved µGrid for cell localization
  • Low skirt format: ideal for immersion microscopy
  • Robust format: stability against focal shift during temperature changes and time lapse studies
  • 12 notch grip zone: easy grip for dish and lid
  • Orientation marks: align dish on microscope stage
  • Imaging Reservoir: reduced volume for staining and labelling
  • Tissue Culture Surface treatment: improved cell spreading, adhesion and growth

 

Applications:

Cytometry, Cell localization and re-localization, Life Cell Imaging, Fluorescence-Correlation spektroscopy (FCS), Confocal Laser Scanning Microscopy (LSM), Total Internal Reflection Fluorescence (TIRF), Differential Interference Contrast (DIC/Nomarski), Fluorescence Resonance Energy Transfer (FRET), Fluorecence Recovery after Photobleaching (FRAP), Weakly fluorescent stains / proteins, Fluorescence In-Situ Hybridisation (FISH), Immune histology

Detailed description: 

Imaging Dish µGrid are equivalent to the Imaging Dish 1.5 (170 µmcover glass bottom) but the cover glass carries in addition a micro grid. This µGrid located in the focus plane of the cover glass. The microgrid supports localisation and re-localisation of the Region Of Interest (ROI) even if no computer assisted motor stage is available. The grid is oriented towards the macroscopic orientation marks of the Imaging Dish. When the Imaging Dish µGrid is combined with the Imaging Dish Stage Frame it is easily possible to navigate to the ROIs at different time points, Even if the Imaging Dishes are removed from the microscope stage in between.

µGrid propertiesThe µGrid is made by precise laser marking. The single grid lines have a width of 3 µm, a length of 9 mm and a depth of 0,3 µm. In widefield and phase contrast illumination the lines are visible but do not influence cell attachment or orientation. In fluorecence mode the grid lines are not visible and do not cause artefacts during the image acquisition.

  

The focus level of the glass area is placed only 400µm above the basement line of the dish body. High quality cover glasses made from borosilicate glass are used. These glass bottoms provide an excellent planicity and focus control and superior imaging qualities.

The macroscopic orientation of the dishes is supported by four marks (N-O-S-W). The combination of engraved microgrids to support microscopic orientation to retrieve the region of interest (ROI) with the twelve corner grip zone of the dishes enables an easy orientation and positioning of the dishes if combined with our Imaging Dish Stage Frame. Therfore the same orientation of the dishes can be established even if the dish has to be removed from the stage in between two observation sesions.

 

Specification

Cover glass properties

 
Thickness

1.5, 170 µm +/- 10%

Glass type borosilicate, hydrolytic class I
Refractive Index 1,52
Abbe's number 50
Light transmission @ 310 nm > 85%
Light transmission @ 360 nm > 90%
Surface quality TC (Tissue Culture Quality)

 

TC= Tissue Culture, aminanted surface, suitable for most adherend cell lines (ze.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (z.B. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.

 

Technical data

 
Article number 7160
Outer dish diameter 35 mm
Diameter cover glass area 18 mm
Dish height 9,4 mm
Distance dish bottom rim to focus plane 400 µm
Planicity < 5 µm
Total dish volume 7 ml
Suggested working volume 2 ml
Suggested cell seeding volume 750 μl
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