Imaging Chamber

Chambered microscope slides with removable chambers

Order information

         
Article number Wells / chamber  Chambers / bag Chambers/ Sales Unit Lid Sterile
8011-16 1 1 / bag 16 /box + +
8011-80 1 1 / bag 80 / box + +
8012-16 2 1 / bag 16 / box + +
8012-80 2 1 / bag 80 / box + +
8014-16
4 1 / bag 16 / box + +
8014-80 4 1 / bag 80 / box + +
8018-16 8 1 / bag 16 / box + +
8018-80 8 1 / bag 80 / box + +


Features:

  • Tool free removable chambers
  • Tissue culture surface microscopy slides
  • High temperature and chemical resistance

Applications:

Life cell imaging, immunohistochemistry, fluorescence in-situ hybridization

Detailed Description:

The high performance polymer of chamber bodies and lids withstands temperatures up to 90°C. Due to this temperature stability the Imaging Chambers show also excellent stability of the focus plane when the chambers are exposed to temperature shifts (e.g. when the chambers are transported from the incubator to the microscope). Furthermore the Imaging Chambers are resistant against many chemicals (e.g. acetone, DMSO, alcohols). Therefore the full products are suitable for in-situ hybridization technologies as well as for a wide range of fixation and staining protocols.
The microscope slides of the Imaging Chamber products are made from 1 mm soda lime glas of higher quality (optimised for fluorescence microscopy) All microscope slides have frosted ends (outside the chambers). All corners of the slides are smoothed.  Imaging Chambers with microscope slides can be used with long distance objectives.

Cell adhesion, spreading and distribution are supported by ower improved surface modifaction known from our Imaging Plate CG products. Imaging Chambers are available in 1 well, 2 well, 4 well and 8 well Format

 

Specification

Microsope slide properties

 
Thickness 1 mm
Glass type Soda lime, smoothed corners, frosted ends
Refractive index 1,52
Abbe's number 50
Length 76 mm
Width 26 mm
Surface quality TC (Tissue Culture Quality)

 

 

TC= Tissue Culture, aminanted surface, suitable for most adherend cell lines (ze.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (z.B. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.

 

Technical data

       
Article number 8011 8012 8014 8018
Number of wells / chamber  1 2 4 8
Chamber height 10 mm 10 mm 10 mm 10 mm
Imaging area / well  10,84 cm2  5,04 cm2  2,14 cm2 0,88 cm2
Total volume / well  11 ml  5,5 ml  2,2 ml 0,9 ml
Suggested working volume / well  2000 µl  1000 µl  500 µl 300 µl
Total chamber length  58 mm  58 mm  58 mm 58 mm
Total chamber width  26 mm  26 mm  26 mm 26 mm

 

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