Imaging Chamber CG

Chambered cover glasses (170µm)

Order information

         
Article number Wells / chamber  Chambers / bag Chambers/ Sales Unit Lid Sterile
8001-16 1 1 / bag 16 /box + +
8001-80 1 1 / bag 80 / box + +
8002-16 2 1 / bag 16 / box + +
8002-80 2 1 / bag 80 / box + +
8004-16
4 1 / bag 16 / box + +
8004-80 4 1 / bag 80 / box + +
8008-16 8 1 / bag 16 / box + +
8008-80 8 1 / bag 80 / box + +


Features:

  • Tool free removable chambers
  • Tissue culture surface borosilicate cover glass
  • High temperature and chemical resistance

Applications:

Life cell imaging, immunohistochemistry, fluorescence in-situ hybridization, super high resolution microscopy, fluorecence correlation spectroscopy, laser scanning microscopy

Detailed Description:

Imaging Chamber CG products are chambered cell culture vessels with a bottom made from 170 µm cover glass. Chambers and lids are made from high performance polymers and are sealed against the glass bottoms by biological inert silicone adhesive. At the end of the experiments the glasses can be detached from the polymer chambers and used for further processing or archiving.

The high performance polymer of chamber bodies and lids withstands temperatures up to 90°C. Due to this temperature stability the Imaging Chabers show also excellent stability of the focus plane when the chambers are exposed to temperature shifts (e.g. when the chambers are transported from the incubator to the microscope). Furthermore the Imaging Chambers are resistant against many chemicals (e.g. acetone, DMSO, alcohols). Therefore the full products are suitable for in-situ hybridization technologies as well as for a wide range of fixation and staining protocols.

The cover glasses of the Imaging Chambers CG are made from 170 µm borosilicate glass compliant to hydrolytic class I. Imaging Chambers CG with cover glass bottoms can be used for high resolution microscopy with immersion objectives (providing a high numerical apperture).

Cell adhesion, spreading and distribution are supported by ower improved surface modifaction known from our Imaging Plate CG products. Imaging Chambers are available in 1 well, 2 well, 4 well and 8 well Format.

 

Specification

Cover glass slide properties

 
Thickness 170 µm +/- 10%
Glass type Borosilicate glass, hydrolytic class I
Refractive index 1,52
Abbe's number 50
Length 58 mm
Width 26 mm
Surface quality TC (Tissue Culture Quality)

 

 

TC= Tissue Culture, aminanted surface, suitable for most adherend cell lines (ze.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (z.B. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.

 

Technical data

       
Article number 8001 8002 8004 8008
Number of wells / chamber  1 2 4 8
Chamber height 10 mm 10 mm 10 mm 10 mm
Imaging area / well  10,84 cm2  5,04 cm2  2,14 cm2 0,88 cm2
Total volume / well  11 ml  5,5 ml  2,2 ml 0,9 ml
Suggested working volume / well 2000 µl 1000 µl  500 µl 300 µl
Total chamber length  58 mm  58 mm  58 mm 58 mm
Total chamber width  26 mm  26 mm  26 mm 26 mm

 

Script logo